THE LABORATORY TESTING OF THE PCR-BASED PROTOCOL OF DETECTION OF THE RABBIT HAEMORRHAGIC DISEASE VIRUS RNA
DOI:
https://doi.org/10.31073/onehealthjournal2024-III-05Keywords:
rabbit haemorrhagic disease virus, risk analysis, epidemiology, diagnostics, rabbits, PCRAbstract
Rabbit Haemorrhagic Disease (RHD), also known as Rabbit Viral Haemorrhagic Disease (RVHD), is a highly contagious and often fatal viral disease that affects domestic and wild rabbits. It's caused by two related viruses: Rabbit Haemorrhagic Disease Virus (RHDV) and Rabbit Haemorrhagic Disease Virus 2 (RHDV2).
The disease is endemic in many European, Asian and American countries, but the agent is still recognized as an emergent infection, associated with mass losses and extremely high mortality in rabbits of all breeds.
One Health Scientific and Research Institute, PSI in collaboration with SRI ‘Veterinary Biotechnologies’, LLC developed the in house PCR-based protocol for RHDV detection and RHDV-2 differentiation, that requires fast implementation. This diagnostics kit evaluation in described under OIE requirements with determination of the sensitivity, specificity, repeatability and domain-specificity. Detection kit is recommended for practical application.
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